Berberine-induced apoptosis in leukemia Molt-4 cell line and expression of NF-κB/p65 and Caspase-3

VernacularTitle:小檗碱诱导白血病Molt-4细胞株凋亡及对NF-κB/p65、Caspase-3表达的影响
Author: Yan JIANG ; Qun HU ; Yejun WANG ; Aiguo LIU ; Xiaoling ZHANG
Publication Type:Journal Article
Keywords: Berberine; Leukemia; Apoptosis; NF-kappaB; Caspase-3
From:Journal of Leukemia & Lymphoma 2012;21(6):342-344
CountryChina
Language:Chinese
Abstract: [Objective] To study the inhibition effect of berberine on cell proliferation in acute T lymphocytic leukemia cell line Molt-4,and its mechanisms.[Methods] The logarithmic growing cell lines were incubated with 0,10,100 μg/ml berberine for various hours.The apoptosis induced by berberine was detected by flow cytometry,eletrophoresis and microscopy.The protein pro-Caspase-3 and p65 were determined by Western bolt assay.[Results]Treated with berberine,Molt-4 cells were found with apoptosis bodies by microscopy.By flow cytometry,it was found that number of G2/M cells was increased and number of S cells was reduced.Necrosis band was found in DNA ladder electrophoresis.The expression of pro Caspase-3 was activated partly and p65 was down-regulated in Molt-4 cells treated with berberine.[Conclusion]Berberine can induce Molt-4 cells apoptosis, and NF-κB、Caspase-3 have been shown to regulate the apoptosis programme.