Effect of apolipoprotein E polymorphisms on expression of p38MAPK in the early period of astrocytes injury

VernacularTitle:载脂蛋白E基因多态性对星形胶质细胞致伤后早期胞内p38MAPK表达的影响
Author: Haitao WU ; Shuai ZHOU ; Haijian XIA ; Yuyu WANG ; Pei WANG ; Xiaochuan SUN
Publication Type:Journal Article
Keywords: Astrocytes; Apolipoproteins; Polimorphism; p38 mitogen activated protein kinase
From:Chinese Journal of Trauma 2014;30(9):962-965
CountryChina
Language:Chinese
Abstract: Objective To investigate the correlation between apolipoprotein E (APOE) polymorphisms and expression of p38MAPK of injured astrocytes in the early stage.Methods Scratch injury to astrocytes of three different alleles of APOE (ε2,ε3,andε4) was induced.RT-PCR and Western-blot were applied to detect dynamic changes of intracellular p38MAPK before injury and at 12,24,48,and 72 hours postinjury.Results Expression of p38MAPK in APOEε2,ε3,andε4 astrocytes increased gradually over time,whereas before and 12-hour after injury,the difference was insignificant in pair comparison (P ＞ 0.05).p38MAPK in APOEε2,ε3,andε4 astrocytes revealed progressive up-regulation at 24,48,and 72 hours postinjury,but the expression in APOEε4 astrocytes was the highest (P ＜ 0.05).Conclusion In the early period of injury,highly expressed p38MAPK in APOEε4 astrocytes indicates a more active p38MAPK-induced inflammatory response in APOEε4 carriers which may contribute to acute exacerbation and poor outcome.