Effect and mechanism of IKK-2 receptor blocker on corneal stromal fibroblasts
10.3969/j.issn.2095-4344.2015.29.019
- VernacularTitle:IKK-2受体阻滞剂对角膜基质成纤维细胞的作用及机制
- Author:
Wei ZHONG
;
Hong JIANG
;
Jing ZHAO
- Publication Type:Journal Article
- Keywords:
lipopolysaccharide;
Fibroblasts;
Dexamethasone
- From:Chinese Journal of Tissue Engineering Research
2015;(29):4688-4694
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Corneal stromal fibroblasts have been shown to express interleukin-8 in the stimulation of lipopolysaccharide. Different reactions of fibroblasts to lipoprotein or other inflammatory mediators may constitute different characteristics of different tissues in the inflammatory response. OBJECTIVE:To observe the inhibitory effect of IKK-2 receptor blocker TPCA-1 on human corneal stromal fibroblasts to secrete inflammatory cytokines, chemokines and cel adhesion molecules under the stimulation of lipopolysaccharide and its signal transduction pathway, to compare with dexamethasone, and to explore alternative or synergistic effects after their combination. METHODS:This study measured the secretion of interleukin-1, tumor necrosis factor alpha, interleukin-6, and interleukin-8 from cultured human corneal stromal fibroblasts under the action of basic state and lipopolysaccharide, and their changes after the intervention with IKK-2 receptor blocker TPCA-1 and dexamethasone. We also detected expression level of intercelular adhesion molecule-1 and interleukin-6 in cel surface, and verified the changes in expression levels of intercelular adhesion molecule-1, interleukin-6, and interleukin-8 from mRNA level, as wel as examined the expression of nuclear factor kappa B under above conditions.
