Mechanism of phloretin induced the apoptosis of hepatoma carcinoma cell SMMC-7721
- VernacularTitle:根皮素诱导肝癌细胞SMMC-7721凋亡的机制研究
- Author:
Hui WANG
;
Handong WU
;
Zheng LIU
- Publication Type:Journal Article
- Keywords:
phloretin;
human hepatoma cells SMMC-7721;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(6):25-28
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract ] Objective To investigate effect of phloretin on apoptotic of hepatoma carcinoma cells SMMC-7721, and explore its mechanisms.Methods Logarithmic phase of hepatoma carcinoma cells SMMC-7721 were cultured separately with 30, 60, 120 mg/L phloretin, morphological alterations of apoptotic were observed by phase contrast microscopy and AO/EB double fluorescence staining method was used to observe were low, medium and high concentration trentment group, respectively.the cells treated by phloretin.Apoptotic rates, cell cycle progression, mitochondrial trans-membrane potential and intracellular calcium homeostasis were detected by flow cytometry.Results Cells appeared typical apoptosis morphological alterations.Phloretin induced SMMC-7721 cell line apoptosis in a dosage and duration dependent manner.Cell cycle was arrested at G1 phase, mitochondrial trans-membrane potential decreased, intracellular free Ca2 +increased.Conclusion Phloretin induce apoptosis of SMMC-7721 by affecting cell cycle progression, reducing mitochondrial trans-membrane potential and changing intracellular calcium homeostasis.
