The Effect of XRCC2 Gene Silencing Combined with Radiation on Human Colonic Transplanted Carcinoma in Nude Mice
- VernacularTitle:XRCC2基因沉默联合辐射对结肠癌细胞裸鼠移植瘤生长的影响
- Author:
Qin WANG
;
Yan WANG
;
Liqing DU
;
Chang XU
;
Feiyue FAN
;
Qiang LIU
- Publication Type:Journal Article
- Keywords:
XRCC2 gene;
radiation;
colonic carcinoma;
BALB/c nude mice
- From:Journal of China Medical University
2015;(5):385-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the curative effect of XRCC2 gene silencing mediated by shRNA combined with radiation on human colonic trans?planted carcinoma in nude mice. Methods Colonic carcinoma T84 cells were transfered into BALB/c nude mice to establish a tumor xenograft mod?el in vivo. Mice were divided into three groups:control,shRNA?SC and shRNA?XRCC2 and exposed to X?ray radiation. The change of volume and weight of the xenografts were examined after receiving radiotherapy and the pathological analysis of tumor tissues were conducted. Results Tumor xenografts transfected with shRNA?XRCC2 in nude mice grew slowly. The xenograft volume in the shRNA?XRCC2 group was decreased significant?ly from day 12 to day 28 after radiotherapy compared with the control group(P<0.01). The xenograft weight in the shRNA?XRCC2 group was small?er than in the control group,with statistically significant difference(t=18.843,P<0.01). The inhibited rate of xenografts in the shRNA?XRCC2 group(56.25%),was markedly higher than that in the shRNA?SC group(4.69%). Pathological analysis of colonic transplanted carcinoma showed that nuclear atypia was not obvious,karyokinesis was decreased and small areas of necrosis were present in tumor xenografts treated with shRNA?XRCC2 transfection. Conclusion XRCC2 gene silencing combined with radiation has significant inhibition effect on colonic transplanted carcino?ma in nude mice.
