Role of 17-AAG in inducing apoptosis and cell cycle arrest of HCT-15 cells
10.3969/j.issn.1000-4718.2015.01.019
- VernacularTitle:17-AAG 阻滞 HCT-15细胞周期并诱导其凋亡
- Author:
Xuerong ZHAO
;
Jianping WANG
;
Lijun XIAO
;
Qian XU
;
Enhong ZHAO
;
Xin ZHENG
;
Huachuan ZHENG
;
Shuang ZHAO
- Publication Type:Journal Article
- Keywords:
17-AAG;
HCT-15 cells;
Apoptosis;
Cell cycle;
STAT3
- From:Chinese Journal of Pathophysiology
2015;(1):98-103
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of 17-AAG on apoptosis and cell cycle of HCT-15 cells and to clar-ify the related mechanisms .METHODS: MTT method was employed to evaluate the inhibitory effects of 17-AAG with Aifferent time and different doses on the proliferation of HCT-15 cells.The cells were stained with Annexin V-FITC/propid-iumiodide and measured by flow cytometry .The expression of STAT3, cyclin D1, Cyt C, caspase 9 and caspase 3 at mR-NA and protein levels was determined by RT-PCR and Western blotting .RESULTS:Treatment with 17-AAG at concentra-tion of 1.25~20 mg/L for 24 h and 48 h significantly inhibited the activity of HCT-15 cells at both time-and concentra-tion-dependent manners .Treatment with 17-AAG at concentrations of 0.425, 0.85 and 1.7 mg/L for 48 h significantly in-duced apoptosis and cell cycle arrest of HCT-15 cells.The exposure of 17-AAG at concentrations of 0.425, 0.85 and 1.7 mg/L for 48 h to the HCT-15 cells significantly down-regulated the expression of STAT 3 and cyclin D1 at mRNA and pro-tein levels, but up-regulated Cyt C, caspase 9 and caspase 3 mRNA and protein in a concentration-dependent manner . CONCLUSION:17-AAG inhibits the cell activity , induces apoptosis and G 1 arrest by down-regulating the expression of cyclin D1, and promoting the mitochondria apoptosis through STAT 3 pathway.
