Effects of electroacupuncture at meridian point on the mRNA expressions of corticotropin- releasing factor and corticosteroid in the hippocampus after cerebral ischemia reperfusion in rats
10.3760/cma.j.issn.1673-4246.2015.03.012
- VernacularTitle:电针经穴对脑缺血再灌注大鼠海马促肾上腺皮质激素释放因子和皮质醇mRNA表达影响
- Author:
Luming REN
;
Yuying CAI
- Publication Type:Journal Article
- Keywords:
Brain ischemia;
Reperfusion injury;
Electroacupuncture;
Corticotropin-releasing hormone;
Glucocorticoids;
Gene expression;
Rats
- From:
International Journal of Traditional Chinese Medicine
2015;(3):235-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of electroacupuncture at meridian point on the mRNA expressions of corticotropin-releasing factor (CRF) and corticosteroid (CORT) in the hippocampus after cerebral ischemia reperfusion in rats.MethodsA total of 150 SD rats were randomly divided into 5 groups by random number table method: normal control group, sham operation group, model group, meridian point electroacupuncture group and non-meridian point electroacupuncture group, with 30 rats in each group. A model of focal cerebral ischemic reperfusion was induced using the modified intraluminal thread method. In the meridian point electroacupuncture group, at 6 h after cerebral ischemia reperfusion, acupuncture “LI11” on both sides, bilateral “ST36”, “GV20”, ”GV16” daily for 7 d. The neurological deficits were evaluated 1 d, 3 d, and 7 d after cerebral ischemia reperfusion, and then all rats were sacrificed and the hippocampi were harvested. The mRNA expressions of CRF and CORT in the hippocampus were determined by quantitative real-time PCR.ResultsThe mRNA expressions of CRF (1.122 ± 0.249, 1.190 ± 0.666, 0.454 ± 0.612 in the model group; 0.021 ± 0.049, 0.021 ± 0.027, 0.035 ± 0.005 in the sham operation group) and CORT (0.917 ± 0.113, 1.024 ± 0.290, 0.709 ± 0.055 in the model group; 0.016 ± 0.013, 0.016 ± 0.006, 0.043 ± 0.006 in the sham operation group) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly increased in the model group compared with the sham operation group (all P<0.01). The mRNA expressions of CRF (0.424 ± 0.104, 0.339 ± 0.476, 0.095 ± 0.021) and CORT (0.377 ± 0.073, 0.138 ± 0.025, 0.158 ± 0.010) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (allP<0.01). The neurological deficits scale 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (1.83 ± 0.75, 1.50 ± 0.55 and 1.17±0.41 in the meridian point electroacupuncture group; 2.50 ± 0.84, 2.33 ± 0.52 and 1.67 ± 0.52 in the model graoup; allP<0.01). Conclusion Electricacupuncture at meridian point can reduce the mRNA expressions of CRF and CORT in the hippocampus, and improve neurological deficits after cerebral ischemia reperfusion in rats.
