Effect of miR-185 * on BDNF/TrkB signaling pathway expression in epileptic neurons
10.7652/jdyxb201502005
- VernacularTitle:miR-185?对癫痫海马神经元中 BDNF-TrkB 通路表达的影响
- Author:
Hao CAI
;
Jiangli LI
;
Wei XIE
;
Wei CHANG
;
Yijun SONG
- Publication Type:Journal Article
- Keywords:
miR-185 ?;
BDNF;
TrkB;
hippocampal neuron;
epilepsy;
Western blot
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2015;(2):169-174
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To study the BDNF/TrkB signaling pathway in the epilepsy model of hippocampal neurons and the regulatory effect of on it.Methods The primary hippocampal neurons cultured in vitro for 7 days were randomly divided into seven groups:control group,epilepsy group,control+BDNF group,epilepsy+BDNF group,control + miR-185 ? group,epilepsy + miR-185 ? group,and epilepsy + miR-185 ? + BDNF group.We constructed miR-185 ? lentivirus vector and observed the changes of BDNF/TrkB pathway expression after transfaction of miR-185 ? by immunohistochemistry,patch clamp technique and Western blot technique.Results Compared with the control+BDNF group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly lower in epilepsy+BDNF group (P < 0.05 )and control group (P < 0.001 ).Compared with the epilepsy group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly higher in epilepsy+BDNF group (P <0.05).Compared with the epilepsy+miR-185 ? +BDNF group,the phosphorylated TrkB (pTrkB)/TrkB value was significantly lower in epilepsy + BDNF group and epilepsy + miR-185 ? group (P < 0.001 ).BDNF could promote the signaling conduction and miR-185 ? could remove the inhibition of BDNF/TrkB signaling.Conclusion BDNF can activate the BDNF/TrkB signaling pathway and transfection with miR-185 ? can relieve the inhibition of BDNF/TrkB signaling pathway of epileptic state by up-regulating the expression of TrkB.
