Neuroprotection of selective antegrade cerebral perfusion on pig model exposed to deep hypothermia circulatory arrest
10.3969/j.issn.1006-5725.2015.05.001
- VernacularTitle:选择性顺行性脑灌注在猪深低温停循环模型中的脑保护机制
- Author:
Ziyou LIU
;
Mengya LIANG
;
Guangxian CHEN
;
Zhixian TANG
;
Jian RONG
;
Jianping YAO
;
Xiao YANG
;
Zhongkai WU
- Publication Type:Journal Article
- Keywords:
Circulatory arrest,deep hypothermia induced;
Selective antegrade cerebral perfusion;
TLR4;
NF-κB;
IL-6;
Brain injuries
- From:
The Journal of Practical Medicine
2015;(5):693-696
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of TLR4/NF-κB pathway in cerebral injury resulting from DHCA ( deep hypothermia circulatory arrest ) as well as the effect of SACP ( selective antegrade cerebral perfusion). Methods Twelve pigs were randomly assigned to DHCA group (n = 6) or SACP group (n = 6) at 18 ℃ for 80 min. IL-6 was assayed by ELISA. Apoptosis and NF-κB proteins were detected by fluorescence TUNEL and Western blot, respectively. The level of TLR4 was determined through qRT-PCR and Western blot. Results Serum IL-6 level of SACP group was significantly lower at the end of circulation arrest and experiment and apoptotic index and NF-κB protein were apparently lower in SACP group (P < 0.05). The level of TLR4 protein and mRNA from SACP group decreased significantly (P < 0.05). Conclusions TLR4/NF-κB pathway plays a critical role in pathogenesis of DHCA cerebral injury and attenuating TLR4/NF-κB cytokines probably contributes to neuroprotection of SACP. TLR4/NF-κB pathway may be a novel target for DHCA.
