Effect of Rosiglitazone on Insulin Resistance and ROS . IKK Signaling Pathway in Vascular Endothelial Cells
10.3870/yydb.2014.11.005
- VernacularTitle:罗格列酮对血管内皮细胞胰岛素抵抗及ROS/IKK的影响
- Author:
Fanghui CHEN
;
Renze YANG
;
Xinhui LUO
;
Sheng ZHONG
;
Zeling LI
;
Taohui ZENG
;
Guilin WEI
- Publication Type:Journal Article
- Keywords:
Rosiglitazone;
Endothelial dysfunction;
Insulin resistance;
Reative oxygen species;
IrB kinase
- From:
Herald of Medicine
2014;(11):1420-1423
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of rosiglitazone on insulin resistance( IR)induced by high glucose in vascular endothelial cells and its possible mechanism. Methods Human umbilical vein endothelial cells( HUVECs) was divided into 3 groups:the normal control group cultivated in DEME medium with 5. 5 mmol·L-1 glucose;the high glucose group( HG)cultivated in DEME medium with 33 mmol · L-1 glucose for 24 h after the IR model was set up;the rosiglitazone group cultivated in DEME medium with 33 mmol·L-1 glucose and 10 μmol·L-1 of rosiglitazone for 24 h after the IR model was set up. The cell viability,nitric oxide(NO),endothelin-1(ET-1),mitochondrial membrane potential,reactive oxygen species ( ROS),p-IKK and IkBa protein levels were detected. Results Compared with the normal control,the cell viability,the level of NO and the mitochondrial membrane potential were decreased,levels of ET-1 and ROS increased,p-IKK expression was up-regulated,and IκBα expression was down-regulated in HG group(all P〈0. 01). Rosiglitazone reversed these changes in a time-dependent manner(P〈0. 05). Conclusion Rosiglitazone has the protective effect on insulin resistance induced by high glucose in vascular endothelial cells via inhibiting ROS/IKK signaling pathway.
