Comparison of Anti-inflammatory and Hepatoprotective Activities of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC
10.3870/yydb.2014.09.007
- VernacularTitle:竹叶柴胡与北柴胡的抗炎保肝作用比较
- Author:
Ting DU
;
Shiming DU
;
Gang WANG
;
Rongjin SUN
;
Guangyi YANG
;
Fang YE
;
Lingling YUAN
;
Tao LIU
- Publication Type:Journal Article
- Keywords:
Bupleurum marginatum Wall. ex. DC;
Bupleurum chinense DC.;
Anti-inflammatory effects;
Hepatoprotective effects
- From:
Herald of Medicine
2014;(9):1144-1149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare anti-inflammatory and hepatoprotective effects of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. Methods The anti-inflammatory effect of two kinds of radix bupleuri decoction were compared using the xylene-induced ear edema mice model and the egg white-induced foot swelling rats model. Acute liver injury in mice was induced through intraperitoneal injection of peanut oil with 0. 1%carbon tetrachloride. Alanine aminotransferase ( ALT ) , aspartate aminotransferase ( AST ) , and alkaline phosphatase ( ALP ) in serum, superoxide dismutase ( SOD ) , malondialdehyde ( MDA ) , and glutathione ( GSH ) in liver tissue homogenate were detected. Histopathological changes, transforming growth factor-β ( TGF-β) and nuclear factor-κB ( NF-κB) in hepatic tissues were also observed. Results Ear swelling experiment showed that the decoction of both Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. had equal anti-inflammatory effects (P>0. 05). Similarly,the decoction of Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. improved levels of ALT,AST,SOD,GSH,and MDA to the same extent compared with the model control group ( P<0. 05 or P<0. 01 ). There was no significant difference among all groups in ALP levels ( P > 0. 05 ). Histopathological and immunohistochemical experiments showed that expressions of TGF-β and NF- κB were similar between the two kinds of radix bupleuri. Conclusion Bupleurum marginatum Wall. ex DC. and Bupleurum chinense DC. have similar anti-inflammatory and hepatoprotective effects.
