Mechanisms of hyperglycemia induced by immunosuppressant FK506
10.3969/j.issn.1000-4718.2014.08.004
- VernacularTitle:免疫抑制剂 FK506所致血糖升高副作用的机制研究
- Author:
Ling ZHANG
;
Meng SUN
;
Bo SHI
;
Lili TANG
;
Cunzao WU
;
Yong CAI
;
Peng XIA
;
Shaoling ZHENG
;
Yirong YANG
;
Bicheng CHEN
- Publication Type:Journal Article
- Keywords:
FK506;
Bloodglucose;
Peroxisomeproliferator-activatedreceptorγ;
Adipocytokines
- From:Chinese Journal of Pathophysiology
2014;(8):1363-1367
- CountryChina
- Language:Chinese
-
Abstract:
[ABSTRACT]AIM:ToinvestigatetheeffectofimmunosuppressantFK506onserumglucoseinratsandtoex-plore its mechanism .METHODS: Sprague-Dawley rats ( n =12 ) were randomly divided into drug group and normal group.The rats in drug group were intraperitoneally injected with FK 506 at dose of 1 mg· kg-1 · d-1 and the rats in nor-mal group received saline (1 mL· kg-1 · d-1 , ip) for 14 d.The fasting weight and fasting glucose were regularly meas-ured every 2 d.Visceral fat was isolated from the rats at the end of experiment .The mRNA expression of adiponectin , lep-tin, visfatin, resistin, retinol-binding protein 4 ( RBP4) and peroxisome proliferator-activated receptors γ( PPAR-γ) was determined by real-time fluorescence quantitative PCR .The protein expression of PPAR-γand adiponectin was measured by Western blotting .RESULTS:Compared with normal group , the concentration of fasting blood glucose in model group was significantly increased from the 10th day (P<0.05).At day 14, the fasting blood glucose of the model group increased from (5.10 ±0.62) mmol/L to (7.73 ±0.73) mmol/L.No significant change of blood glucose in normal group between the 10th day and the 14th day [from (4.66 ±0.32) mmol/L to (5.80 ±0.10) mmol/L] was observed.Compared with normal group , the mRNA expression of PPAR-γ, adiponectin and leptin in the adipose tissue of model group was signifi-cantly decreased ( P <0.01 ) , whereas the expression of visfatin , resistin and RBP4 was significantly increased ( P <0.05).Compared with normal group, the expression of PPAR-γand adiponectin in model group was decreased (P <0.01).CONCLUSION:FK506 may decrease the expression of PPAR-γto change the expression of adipocytokines and induce hyperglycemia in rats .
