Study on the protecting function of Berberine on the invasion of Streptococcus pneumoniae to A549 cells in vitro
10.3969/j.issn.1006-5725.2014.16.005
- VernacularTitle:小檗碱对肺炎链球菌侵袭A549细胞的保护作用
- Author:
Rong WANG
;
Banglao XU
- Publication Type:Journal Article
- Keywords:
Streptococcus pneumonia;
TypeⅡpneumocyte;
Invasion
- From:
The Journal of Practical Medicine
2014;(16):2538-2539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether Berberine can inhibit the invasion of Streptococcus pueum oniae ( S.pn) to A549 cells in vitro. Methods A549 cells were pretreated with the different concentrations of Berberine and incubated together with S.pn. A549 cells were lysed with 0.025%Triton X-100.Diluted lysate was streaked on TSAB plate and incubated overnight. The colonies on the plate were counted. Results The counts on the plates were 52 ± 21,29 ± 11,13 ± 10,0,0 per well when the concentration of Berberine was 0,25,50,100,200μg/mL, respectively. No invasion was observed when the concentration of Berberine was 100 μg/mL. Conclusion Berberine can inhibit the invasion of S.pn to A549 cells in dose-dependent maner in vitro.
