Expression of the receptor for advanced glycation end products in mice with chronic adriamycin-induced cardiotoxicity
10.3969/j.issn.0529-1356.2014.04.020
- VernacularTitle:晚期糖基化终产物受体在小鼠慢性阿霉素心脏毒性模型中的表达
- Author:
Shengfeng SHI
;
Jinzhou ZHU
;
Ruiyan ZHANG
- Publication Type:Journal Article
- Keywords:
Adriamycin;
Receptor for advanced glycation end products;
Myocardium;
Echocardiography;
HE staining;
Immunohistochemistry;
Western blotting;
Mouse
- From:Acta Anatomica Sinica
2014;(4):550-554
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of receptor for advanced glycation end products ( RAGE ) in chronic adriamycin ( ADR )-induced cardiotoxicity mice . Method A mouse model of chronic cardiotoxicity was established by intraperitoneal injection of 5 mg/kg ADR once a week for continuous 3 weeks.Mice treated with the same volume of saline were used as control group (6 mice in each group).Echocardiography and histopathological examination were used to evaluate the myocardial injury , cardiac function and ventricular remodeling .The expression of RAGE in cardiac tissues was detected by immunohistochemical staining and Western blotting .Results Echocardiography indicated that ADR-treated mice had reduced left ventricular ejection fraction , while histopathological analysis of cardiac tissues showed obvious cardiac damage . RAGE protein in cardiac tissues was highly expressed in chronic ADR-induced cardiotoxicity mice .Conclusion RAGE is highly induced and may be involved in the cardiotoxicity after ADR treatment .
