Effects of Apigenin on Platelet Derived Growth Factor-induced Migration of Vascular Smooth Muscle Cells

VernacularTitle:芹菜素对血小板源生长因子诱导血管平滑肌细胞迁移的影响
Author: Hongjing GUAN ; Changping CUI ; Jiyou HUANG ; Fen SHU
Publication Type:Journal Article
Keywords: Apigenin; Platelet derived growth factor; Vascular smooth muscle cell; Cell movement
From: Herald of Medicine 2014;(10):1265-1268
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of apigenin on the migration of vascular smooth muscle cells (VSMC) induced by platelet derived growth factor (PDGF)-BB and the possible molecular mechanism. Methods VSMCs were isolated from thoracic aortas of male Sprague-Dawley rats using enzyme digestion method. Migration of VSMCs was determined by transwell assay. Western blotting was carried out to evaluate phosphorylation of c-jun N-terminal kinase (JNK). Results Treatment with PDGF-BB (20 ng·mL-1 ) significantly promote VSMC migration,the number of migrated cells was 2. 46 times than that of control group. However,after 12. 5 μmol·L-1 apigenin pretreatment,the number of migrated cells was 46. 5% of the PDGF-BB group. Various dose of apigenin can significantly inhibit VSMC migration induced by PDGF-BB,12. 5 μmol · L-1 apigenin treatment significantly inhibited PDGF-BB phosphorylation of JNK. Conclusion Apigenin can suppress the migration of VSMC induced by PDGF-BB. These beneficial effects on VSMC were at least partly mediated by the inhibition of activity of JNK.