Role of JAK2/STAT3-regulated prohibitin in cardioprotection of H2 S postconditioning in hypoxia/reoxygenation-treated cardiomyocytes
10.3969/j.issn.1001-1978.2014.08.017
- VernacularTitle:JAK2/STAT3调节抗增殖蛋白表达在H2 S后处理心肌细胞中的保护作用
- Author:
Hongya MAO
;
Jieqiong YANG
;
Yong JI
- Publication Type:Journal Article
- Keywords:
hydrogen sulfide;
postconditioning;
hy-poxia/reoxygenation;
myocardial protection;
JAK2/STAT3 signaling pathway;
prohibitin
- From:
Chinese Pharmacological Bulletin
2014;(8):1122-1126
- CountryChina
- Language:Chinese
-
Abstract:
Aim ToinvestigatewhethertheJAK2/STAT3 signaling pathway regulates prohibitin expres-sion to protect cardiomyocytes against hypoxia/reoxy-genation injury in hydrogen sulfide postconditioning. Methods Primaryculturedcardiomyocytesfromneo-natal rats were divided into 6 groups: control group ( Normal) , hypoxia/reoxygenation group ( H/R ) , hy-drogen sulfide postconditioning group ( NP) , hydrogen sulfide with AG490 group ( N + A ) , AG490 group ( AG) , DMSO group ( DMSO) . The survival percent-age of cardiomyocytes and the release of LDH were tested at pre-hypoxia and reoxygenation 2h. After reox-ygenation, cell apoptosis was detected by flow cytome-try. The expression of t-STAT3, p-SATAT3 and PHB were determined with Western blot analysis. Results No obvious changes were observed among the groups before hypoxia (P <0. 05). After reoxygenation 2h, compared with H/R group, NP group significantly im-proved the survival rate of cardiomyocytes ( P <0. 05 ) , inhibited the release of LDH and the myocardi-al apoptosis ( P <0. 05 ) , meanwhile up-regulated the p-STAT3 and PHB expression. However, AG490 abol-ished the cardioprotection offered by hydrogen sulfide postconditioning and the increase in p-STAT3 and PHB expression.Conclusion Hydrogensulfidepostcondi-tioning may protect cardiomyocytes against hypoxia/reoxygenation injury through the JAK2/STAT3 pathway upregulating the expression of prohibitin.
