Effect of curcumin on superantigen TSST-1 induced inflammatory cytokines by splenocytes
10.3969/cjz.j.issn.1002-2694.2014.07.013
- VernacularTitle:姜黄素抑制超抗原 TSS T-1诱导脾细胞炎症因子的作用
- Author:
Xiaoting WEN
;
Yuanming HUANG
;
Zhihao WANG
;
Zhihong REN
;
Shan LU
- Publication Type:Journal Article
- Keywords:
TSST-1;
curcumin;
inflammatory shock;
inflammatory cytokine
- From:
Chinese Journal of Zoonoses
2014;(7):727-732
- CountryChina
- Language:Chinese
-
Abstract:
In the present study ,we aimed to observe the effect of curcumin on TSST-1-induced inflammatory cytokines in splenocytes of mouse and provide evidence for the further study on the effect of curcumin on inflammatory shock .Lactate de-hydrogenase (LDH) release assay was used to determine cytotoxicity of different doses of TSST-1 and curcumin .Inflammatory cytokines were determined by ELISA and flow cytometry .The doses of TSST-1 and curcumin we used in the present study did not cause significant cytotoxicity .TSST-1 induced higher level of IFN-γand IL-2 production but relatively lower level of TNF-α.The production of IL-1β,IL-6 and IL-12 was undetectable .TSST-1 induced Th1 cytokines (IFN-γand IL-2) were not IL-12-dependent which was different from LPS-induced IFN-γ.Curcumin significantly reduced IFN-γand TNF-αproduction at the concentration of 15 umol/L (P<0 .05) ,but had no effect on IL-2 production (P>0 .05) .It’s suggested that curcumin could significantly inhibit the production of IFN-γand TNF-αby splenocytes induced by TSST-1 ,but could not affect the prolifera-tion of T cells .
