Effects of Bmi-1 gene suppression on nasopharyngeal carcinoma cell biology behavior

VernacularTitle:抑制Bmi-1基因表达对鼻咽癌细胞生物学行为的影响
Author: Haiyu LI ; Xingfeng CHEN ; Siying YU ; Geli LIU ; Fangzhou SONG
Publication Type:Journal Article
Keywords: Bmi-1; RNA interference; invasion; mi-gration; cycle; proliferation; apoptosis
From: Chinese Pharmacological Bulletin 2014;(6):833-837
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of small inter-ference RNA-mediated silcencing of the Bmi-1 gene on cell invasion and metastasis in human nasopharyngeal carcinoma cell line CNE-1 . Methods Chemically syn-thesized siRNA targeting the Bmi-1 gene was transfect-ed into CNE-1 cells, which had high invasive and me-tastatic potential. The expression of Bmi-1 mRNA and protein were detected by quantative Real-time PCR and Western blot, respectively. The effects of Bmi-1 knockdown on CNE-1 cells migration and invasion were analysied by Transwell migration assay and Matrigel in-vasion assay. Results Transfected with Bmi-1 siRNA significantly down-regulated the expression of Bmi-1 mRNA and protein as compared with the control group. CNE-1 cells transfected with Bmi-1 siRNA had lower levels of invasion and migration capacity than cells in the control group. Conclusion SiRNA-media-ted silencing of the Bmi-1 gene could significantly in-hibit cell migration and invasion in human nasopharyn-geal carcinoma cell line CNE-1 .