Effect of Gambogic acid on proliferation and invasion in colon cancer LoVo cell line
10.3760/cma.j.issn.1673-4246.2014.03.008
- VernacularTitle:藤黄酸对结肠癌LoVo细胞增殖和侵袭能力的影响
- Author:
Shuwen LIAO
;
Ping YANG
;
Tong ZHANG
;
Xiwen CHEN
;
Qiang WANG
;
Wanglin LI
;
Jie CAO
- Publication Type:Journal Article
- Keywords:
Gambogic acid;
LoVo cells;
Colon cancer;
Invasion
- From:International Journal of Traditional Chinese Medicine
2014;36(3):220-222
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of gambogic acid on proliferation,invasion and protein expression of MMP-2 in LoVo human colon cancer cell line.Methods The CCK-8 assay was used to determine the anti-proliferation ability.Transwell chamber assay was used to study the invasion of LoVo colon cancer cells.Western Blot was applied to examine the protein expression of MMP-2.Results CCK-8 assay showed that after cells treated with 1,2,4 μmol/L GA for 24 h,48 h the inhibition rates were(0.16±0.11)％,(0.24±0.08)％,(0.58±0.01)％,(0.67±0.03)％,(0.79±0.01)％ and (0.27±0.05)％,(0.69±0.09)％,(0.85±0.01)％,(0.87±0.01)％,(0.89±0.01)％,and there had statistically significant differences between control group (0 μmo/L) with experimental group (P＜0.05).Gambogic acid can effectively inhibit LoVo cells invasion at a low-dose concentration,which the numbers of cells that digested the matrigel in control group (0 μmo/L)was 120.50± 8.69 and 69.83 ±4.75 in experimental group (1 μnol/L),P＜ 0.05).Western Blot revealed that Gambogic acid can down-regulate the expression of MMP-2,which the levels of down-regulating the expression of MMP-2 after treated with 1,2,4 umol/L GA were 48.67％,74.72％and 82.70％ (P＜0.05) Conclusion Gambogic acid can effectively inhibit the growth and invasion of the LoVo cells,which may be related with down-regulating the expression of MMP-2.
