Determination of Berberine Hydrochloride and Phellodendrine in Lishukang Capsules by HPLC

VernacularTitle:HPLC测定利舒康胶囊中盐酸小檗碱及黄柏碱含量
Author: Xueyan BI ; Shanshan GUO ; Wenjing CAO ; Qingbo ZHANG
Publication Type:Journal Article
Keywords: Lishukang Capsules; content determination; berberine hydrochloride; phellodendrine
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(2):72-74
CountryChina
Language:Chinese
Abstract: Objective To establish a method for the determination of berberine hydrochloride and phellodendrine in Lishukang Capsules by HPLC. Methods Waters Symmetry C18 column (4.6 mm× 250 mm, 5μm) was used, the mobile phase was acetonitrile-0.1%phosphoric acid (0.1 g sodidum dodecyl sulfonate in 100 mL water), with the flow rate of 1 mL/min by gradient elution. The detection wavelength was set at 284 nm. Results Berberine hydrochloride showed good linear relationship (r 2=0.999 1) in the range of 403.4-10 084.7 μg, the average recovery rate was 99.8%(RSD=1.7%). Phellodendrine showed good linear relationship (r 2=0.999 2) in the range of 169.137 6-4228.44 μg, the average recovery rate was 95.5%(RSD=3.2%). Conclusion The method is specific, accurate and convenient to be used for the quality control of Lishukang Capsules.