Unilateral olfactory functional deprivation model in the left peripheral nostrils by electric cautery injury:olfactory bulb neurogenesis and transformation
10.3969/j.issn.2095-4344.2014.02.012
- VernacularTitle:电烙铁损伤左侧外周鼻孔区构建单侧嗅觉剥夺模型：嗅球神经的发生和转化
- Author:
Jianjun ZENG
;
Xu HE
;
Xiaoxin YAN
;
Xuegang LUO
;
Aihua PAN
- Publication Type:Journal Article
- Keywords:
olfactory sensation;
olfactory bulb;
microtubular proteins;
calbindin,vitamin D-dependent;
parvalbumin
- From:Chinese Journal of Tissue Engineering Research
2014;(2):231-238
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Olfactory bulb neurogenesis, transformation and maturation have been considered the hot topic. Olfactory bulb experience and nervous activity can influence olfactory bulb neurogenesis. However, no study reports that olfactory bulb functions can affect olfactory bulb neurogenesis in guinea pigs.
OBJECTIVE:To investigate the effect of unilateral olfactory functional deprivation on doublecortin, calbindin and parvalbumin expression in olfactory bulb of juvenile guinea pigs.
METHODS:Total y 24 guinea pigs were randomly divided into two groups, which were kil ed after establishing olfactory functional deprivation model through electric cautery injury at the left peripheral nostrils. At 3 and 6 weeks after modeling, the specimens were harvested. The expression change of doublecortin, calbindin and parvalbumin in two sides’ olfactory bulb of juvenile guinea pigs was detected by immunohistochemistry.
RESULTS AND CONCLUSION:The number of doublecortin, calbindin and parvalbumin positive cells in olfactory bulb at the un-deprived side was significantly higher than that at the deprived side at 3 and 6 weeks (P<0.05). This finding indicates that olfactory neural activities can affect neurogenesis and transformation in guinea pigs.
