Different cardioprotective function of transplantation of marrow mesenchymal stem cells from coronary heart disease patients with diabetes mellitus or not to acute myocardial infarction rats
10.3760/cma.j.issn.1001-4497.2014.02.007
- VernacularTitle:糖尿病与非糖尿病冠心病患者骨髓间充质干细胞治疗大鼠急性心肌梗死的比较
- Author:
Yu LIU
;
Tao LIU
;
Xiaodong XUE
;
Dengshun TAO
;
Hui JIANG
;
Huishan WANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Rats;
Myocardial infarction;
Mesenchymal stem cells;
Apoptosis
- From:Chinese Journal of Thoracic and Cardiovascular Surgery
2014;30(2):86-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the different cardioprotective function of transplantation of marrow mesenchymal stem cells(BMSCs) from coronary heart disease(CHD) patients with diabetes mellitus (DM) or not to myocardial infarction rats.Methods 45 adult male rats with acute myocardial infarction experimentally were randomized into three groups,15 rats in each group.BMSCs from CHD patients with DM or not were injected into the infarcted myocardium.In control group,culture medium was used.Echocardiography,Western-blot analysis,TUNEL were performed 4 weeks after transplantation.Results Proliferation of BMSCs in DM group was significantly lower than that in non-DM group.Transplantation of BMSCs in the infarcted border zone improved cardiac function,Bcl-2 protein level in DM and non-DM group compared with control group,moreover,BAX protein level and TUNEL positive cells were decreased.Furthermore,compared with non-DM group,cardiac function and Bcl-2 protein level were decreased,however,BAX protein and TUNEL positive cells were improved.Conclusion BMSCs from CHD patients have proliferative properties.Transplantation of BMSCs can improve cardiac function by inhibiting apoptosis.But DM may decrease apoptosis resistance,which might impair the cardioprotective function of cells transplantation.
