Effects of TSA on Cerebral ischemia/reperfusion injury via JAK/STAT signal pathway in rats
10.3760/cma.j.issn.1671-0282.2014.02.013
- VernacularTitle:曲古抑素A通过JAK/STAT信号通路对脑缺血-再灌注损伤的影响
- Author:
Zhen JIA
;
Zewei ZHANG
;
Qiang GAO
- Publication Type:Journal Article
- Keywords:
Trichostatin-A;
JAK/STAT signal pathway;
Cerebral ischemia/reperfusion injury;
JAK2;
p-JAK2;
Histone deacetylase inhibitor;
Cerebral protection;
Intracellular signal protein
- From:
Chinese Journal of Emergency Medicine
2014;23(2):174-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of trichostatin-A (TSA) on cerebral ischemia/reperfusion injury via Janus kinase/signal transducer and activator of transcription (JAK/STAT) signal pathway.Methods 36 male SD rats were randomly (random number) divided into 3 groups:shamoperated group,ischemia/reperfusion (I/R) group and TSA group.Rat model of middle cerebral artery occlusion/reperfusion (MCAO) was established using a modified filament method.No occlusion was applicated to the sham-operated group.TSA group was injected with TSA 0.05 mg/kg via penile vein,20 minutes before operation.Reperfusion was carried out 24 hours after modeling.Longa 5 score was used to assess the neurological function,and TTC staining was applied to calculate the percentage of cerebral infarction area,The expression of JAK2 and p-JAK2 proteins was detected by Elisa.Results The low expression of JAK2 was observed in each group,and there was no statistical difference between groups (P =0.266).Compared with I/R group,TSA group had lower score in cerebral ischemia-reperfusion injury assessment (P=0.019),smaller area of cerebral infarction (P ＜0.01),reduced expression of p-JAK2 (P =0.009),all of which were of significant difference.Condusions TSA can reduce the cerebral ischemia/reperfusion injury via JAK/STAT signal pathway by down regulating p-JAK2 expression.
