The mechanism of miR-181c induced neuroprotection by hypoxia preconditioning in rats
10.3760/cma.j.issn.1006-7876.2013.11.008
- VernacularTitle:miR-181c对缺氧预处理大鼠卒中的脑保护作用机制
- Author:
Xiaofang HU
;
Shizheng WU
;
Shukun ZHANG
;
Zhenzhong BAI
- Publication Type:Journal Article
- Keywords:
Ischemic preconditioning;
Infarction,middle cerebral artery;
MicroRNAs;
Electron transport complex Ⅳ;
Rats
- From:
Chinese Journal of Neurology
2013;46(11):751-754
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effect of miR-181c on hypoxia-preconditioned ischemia in rats and its mechanism.Methods Thirty-nine male SD rats were randomly divided into 5 groups of control group,sham-operated group,middle cerebral artery occlusion (MCAO)group,hypoxia-preconditioned group,hypoxia-preconditioned and MCAO group.Infarct volume and behavioral deficits were quantified.Real-time PCR was applied to detect the expression levels of miR-181c and Western blotting was used to verify the target protein of mt-cox1.Results Under the treatment of hypoxia-preconditioned,the neurological impairment was alleviated and the infarct volume was reduced significantly from 22.50％ ±2.96％ to 16.40％ ±3.13 ％ (t =5.26,P ＜0.01).The expression of miR-181c was decreased significantly in hypoxia-preconditioned and MCAO group than that in MCAO group (1.89 ± 0.14 vs 3.05 ± 0.26,t =6.10,P ＜ 0.01),and the expression of mt-cox1 protein was also significantly decreased (0.54 ± 0.07 vs 0.93 ± 0.04,t =8.01,P ＜ 0.01).Conclusion Hypoxia-preconditioned may attenuate the ischemic injury in SD rats,which may be related to the down-regulation of the expression of miR-181c,therefore increasing the expression of its targeted protein mt-cox1.
