Effect of genistein on the expression of Bax and Bcl-2 protein in PC12 cells transfected with mutant APP695 type
10.3760/cma.j.issn.1674-6554.2013.09.007
- VernacularTitle:三羟基异黄酮对APP695基因转染PC12细胞Bax和Bcl-2蛋白表达的影响
- Author:
Zhenyu DING
;
Cuixiang ZHAO
;
Fengbin WANG
;
Bing LIU
;
Chunyan TAN
;
Yanan WANG
- Publication Type:Journal Article
- Keywords:
Genistein;
APP695 mutant type;
Bax protein;
Bcl-2 protein;
PC12 cell
- From:Chinese Journal of Behavioral Medicine and Brain Science
2013;22(9):791-793
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of genistein on the expression of Bax and Bcl-2 protein in PC12 cells transfected with mutant APP695 Type.Methods PC12 cells were transfected with pIRES2-EGFP plasmid or pIRES2-EGFP/APP695MT expression plasmid,and then divided into normal control group,Unladen group,APP695 transfected group,GST treatment group(5 μmol/L,15 μmol/L).The Bax and Bcl-2 protein expression in PC12 cells was measured by Immunocytochemistry SABC and Western-blot.Results Immunocyto-chemistry SABC showed that:compared with the normal control group,the expression of bax protein in APP695 transfected group was strongly increased((78.02 ± 1.26) ％,P＜0.01)) and the expression of bcl-2 protein was very weak oppositely ((15.40 ± 0.72) ％,P＜0.01)).Compared with APP695 transfected group,the expression of bax protein in cells of 15umol/L GST treatment group was significantly decreased((22.35 ± 0.49) ％,P＜0.01))and the expression of bcl-2 protein in these cells was strongly increased ((68.11 ± 0.24) ％,P ＜0.01)).Western-Blot showed that:compared with the normal control group,the expression of bax protein in APP695 transfected group was markedly increased and the expression of Bcl-2 protein was significantly decreased.Compared with the APP695 transfected group,the expression of Bax protein was markedly decreased and the expression of Bcl-2 protein was significantly increased in the cells of the GST treatment groups.Conclusion GST can reduce the expression of bax protein and the increased expression of Bcl-2 protein has protective effect on PC12 cells transfected with APP696MT.
