Study of creatine phosphokinase with dried blood spot determination
10.3969/j.issn.1000-3606.2009.11.023
- VernacularTitle:干血滤纸片法测定血清肌酸激酶实验研究
- Author:
Jie WANG
;
Zhiping WANG
- Publication Type:Journal Article
- Keywords:
dried blood spot determination;
creatine phosphokinase;
ROC curve
- From:
Journal of Clinical Pediatrics
2009;(11):1085-1088
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish dried blood spot determination for quantitative analysis of creatine phosphokinase (CK) activity; To analyze the correlation of CK value between serum detection and dried spot determination, and set up a 'cut-off point' for screening of pathologically high CK values in dried blood spot determination. MethodsA total of 100 samples were analyzed quantitatively with two methods (serum detection and dried blood spot determination) for CK activity. The correlation of the two methods was studied. The ' cut-off point' for screening of pathologically high CK in dried blood spot determination was calculated with receiver operating characteristic curve (ROC curve). ResultsOf the 100 samples, 50 were normal and other 50 were abnormal when the 'cut-off point' was set at serum CK of 170 IU/L. There was a positive correlation in CK values between serum detection and dried blood spot determination (r = 0.764, P < 0.01). However, the values measured with the two methods could not be interchanged. The sensitivity and the specificity were 90% when the 'cut-off point' was set at 150 lUlL. The sensitivity and the specificity were 70% and 72%, respectively, when the 'cut-off point' was set at 400 IU/L. ConclusionsThere was a good correlation in CK activity between serum detection and dried blood spot determination. 400 IU/L could be suggested as the 'cut-off point' for detecting pathologically high CK in dried blood spot determination.