Development of the Devices for Synthetic Biology of Glycyrrhizin at an Early Stage：Cloning and Sequencing Analysis of Farnesyl-Diphosphate Synthase Genes in Glycyrrhiza uralensis

VernacularTitle:甘草酸合成生物学元件初探：甘草法呢基焦磷酸合酶基因的克隆及序列分析＊
Author: Ying LI ; Yunyun NIU ; Jingyuan SONG ; Hongmei LUO ; Qiushi LI ; Chao SUN
Publication Type:Journal Article
Keywords: Glycyrrhiza uralensis; Farnesyl-diphosphate synthase; glycyrrhizin; gene clone
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(3):355-359
CountryChina
Language:Chinese
Abstract: Glycyrrhizin is the major bioactive compound in Glycyrrhiza uralensis. Farnesyl-diphosphate synthase (FPS) is one of the major enzymes involved in the glycyrrhizin biosynthetic. We cloned the full length of FPS coding sequence based on the transcriptome data of G. uralensis. The FPS gene of G. uralensis (GlyurFPS) is 1029 bp, coding 342 amino acid. The homology between the sequence of its protein and that of the FPS in Cicer arietinum, Medicago sativa, Medicago truncatula, Glycine max, Lotus japonicus was of 94%, 94%, 93%, 93% and 92%, respectively. Plant FPS gene is very conservative, therefore the phylogenetic tree is same with the plant classification consistent.