Regulation of activated genes of fibroblasts and their improvement of skin aging
10.3760/cma.j.issn.1671-0290.2013.01.013
- VernacularTitle:成纤维细胞激活基因的调控及改善皮肤老化的研究
- Author:
Yuqing QI
;
Tianhang LI
;
Liyun FAN
;
Quanzhong LIU
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinase-1 (MMP-1);
Hyaluronic acid synthetase-2 (HAS-2);
Fibroblast;
Skin aging
- From:Chinese Journal of Medical Aesthetics and Cosmetology
2013;(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanisms using different chemicals to improve the skin aging by the effect of estrogen,vitamin E,vitamin C on the transcriptional level of MMP-1 and HAS-2 mRNAs in cultured human skin fibroblast in vitro.Methods The different concentrations of estrogen,vitamin E,vitamin C were put into cultured human skin fibroblasts.After 24 hours,RT-PCR was used to detect the effects of the different chemicals on the transcriptional levels of the HAS-2 and MMP-1 mRNAs in fibroblasts.Results The effects of estrogen and vitamin C on the MMP-1 mRNA had significant difference between the high dose group and blank control group,and between the low dose group and blank control group (P＜0.05) ; the difference between the low dose group and high dose group was not statistically significant (P＞0.05).The effects of vitamin E and vitamin C on Has-2 mRNA also had significant difference between the high dose group and the blank control group,and between the low dose group and blank control group (P＜0.05),but the difference between the low dose group and high dose group was not statistically significant (P＞0.05).Conclusions Estrogen and vitamin C can reduce the transcription of MMP-1 gene,which decreases the degradation of collagen in the skin; vitamin E and vitamin C can increase the transcription of HAS-2 gene,which may possibly increase the synthesis of hyaluronic acid to alleviate the skin aging.
