Effect of calpain inhibitor on hyperglycemia-induced apoptosis in cultured rat cardiomyocyte
10.3760/cma.j.issn.1008-1372.2012.10.017
- VernacularTitle:钙激活蛋白酶抑制剂对高糖诱导乳鼠心肌细胞凋亡的作用机制
- Author:
Wenzhong WANG
;
Yurong CHEN
;
Zhenwang TANG
- Publication Type:Journal Article
- Keywords:
Myocardium/pathology;
Apoptosis/drug effects;
Carbohydrates/pharmacology;
Peptide hydrolases
- From:
Journal of Chinese Physician
2012;(10):1354-1357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of inhibitor of calpain on hyperglycemia-induced apoptosis in cultured rat cardiomyocyte.Methods Cardiomyocytes were randomly divided into three groups (control,high glucose,and ALLN).MTT assay was used to detect the viability of cultured cardiomyocytes.Laser confocal microscopy was used to observe the mitochondrial permeable transition and membrane potential.The change of Caspase-3 activity in cardiomyocytes was detected by western blot.Results MTT assay showed that,after 72 h of hyperglycemia,the viability of cardiomyocytes was significantly declined (55％ ± 11％),and the viability in the ALLN pretreatment group was (70％ ± 15％) (P ＜0.05).After hyperglycemia,the mitochondrial permeable transition of cardiomyocyte was increased (30％ ± 15％ vs 60％ ± 11％,P ＜0.05),and membrane potential was declined.Hyperglycemia could increase the expression of cleaved capsase-3,while with pretreatment of ALLN the expression of cleaved caspase-3 was downregulation(0.42 ± 0.11 vs 0.21 ±0.12,P ＜0.05).Conclusions The calpain inhibitor can protect cardiomyocytes from apoptosis under the high glucose condition.
