Renal protective effect of lentivirus-mediated adiponectin gene transfection on streptozocininduced early diabetic nephropathy mice
10.3760/cma.j.issn.1001-7097.2012.07.003
- VernacularTitle:脂联素基因重组慢病毒对早期糖尿病肾病小鼠肾脏的保护作用
- Author:
Yanxin SU
;
Huacong DENG
;
Jian LONG
;
Mingxiang ZHANG
;
Zhougui PENG
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathies;
Adiponectin;
Cell proliferation;
Lentivirus
- From:Chinese Journal of Nephrology
2012;28(7):518-523
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the renal protective effect of recombinant lentivirus encoding adiponectin gene on streptozocin-induced early diabetic nephropathy(DN) mice,and to explore its potential mechanism.Methods Forty C57BL/6 mice were randomly divided into normal control group(NC group,n=10),diabetic nephropathy group(DN group,n=10),LentiIRES-EGFP treatment group(DL group,n=10) and Lenti-Acdc-IRES-EGFP treatment group (DA group,n=10).After 8 weeks of recombinant lentivirus injection,kidney to body weight ratio (KW/BW),mean glomerular volume(MGV),fractional mesangial area(FMA),24 h urinary protein excretion(UTP),Scr,BUN,serum albumin and adiponectin were measured.Renal pathological changes were evaluated by electron microscopy.Proliferation of glomendar and tubulointerstitial cells was assessed by immunohistochemistry using PCNA antibody.The phosphorylation of AMP-activated protein kinase(AMPK) and mammalian target of rapamycin protein(mTOR) were detected by Western blotting.Results Adiponectin was successfully over-expressed in STZ-induced DN mice after lentivirus injection.KW/BW,MGV,FMA and UTP were significantly decreased in DA group as compared to DN group and DL group(P＜0.05),but were increased as compared to NC group(P＜0.05).DA group animals had significantly fewer PCNA-positive cells than DN group and DL group(P＜0.01).DA group mice had higher p-AMPK level and lower p-mTOR level as compared to DN group and DL group(P＜0.01).Conclusion Over-expression of adiponectin has beneficial effect on early DN and attenuates aberrant proliferation of renal cells via AMPKmTOR pathway.
