Effect of basic fibroblast growth factor-polylactide sustained release nanospheres on proliferation and adipogenic induction of adipose-derived stem cells in vitro
10.3760/cma.j.issn.1671-0290.2012.02.015
- VernacularTitle:体外碱性成纤维细胞生长因子聚乳酸纳米缓释微球对人脂肪干细胞增殖和成脂分化的影响
- Author:
Pengfei CHA
;
Jianhua GAO
;
Yang CHEN
;
Feng LU
- Publication Type:Journal Article
- Keywords:
Adiposc-derived stem cells;
Basic fibroblast growth factor;
Release nanospheres;
Adipogenic differentiation
- From:Chinese Journal of Medical Aesthetics and Cosmetology
2012;18(2):132-135
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of basic fibroblast growth factor-polyactide release nanospheres on proliferation and adipogenic induction of adipose-derived stem cells in vitro.MethodsAdipose-derived stem cells were isolated and induced for three-line differentiation in vitro.The culture medium and inductive medium of stem cells were prepared containing 0,1,2,3,4 and 5 mg/ml basic fibroblast growth factor-polyactide release nanospheres,respectively.Adipose-derived stem cells were cultured ina 96-well plate and replaced the culture medium containing release nanospheres the second day.The cells proliferation was detected by the method of MTT every day and quanti fication of oil red O every other day.The data obtained were analyzed with SPSS13.0 statistical software.ResultsThe basic fibroblast growth factor polyactide release nanospheres had the ability promoting proliferation and adipogenic induction of adipose-derived stem cells.The best concentration of nanospheres was 3 mg/ml and 4 mg/ml,respectively.ConclusionsThe basic fibroblast growth factor-polyactide release nanospheres could promote proliferation and adipogenic induetionof adipose-derived stem cells significantly.It could be used as an ideal cytokine release nanospheres in adipose tissue engineering.
