Protective effect of icariin on renal ischemia-reperfusion injury in rats and action mechanism

Guiwen Feng; Wenjun Shang; Yue Wang; Xinlu Pang; Jinfeng LI; Lei Liu

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Protective effect of icariin on renal ischemia-reperfusion injury in rats and action mechanism

VernacularTitle:淫羊藿苷减轻大鼠肾脏缺血再灌注损伤的作用及机制研究
Author: Guiwen FENG ; Wenjun SHANG ; Yue WANG ; Xinlu PANG ; Jinfeng LI ; Lei LIU
Publication Type:Journal Article
Keywords: Ischemia reperfusion injury; Oxidoreductases; Icariin; Kidney; Rats
From: Chinese Journal of Organ Transplantation 2012;33(9):559-562
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of icariin on renal ischemia-reperfusion injury (IRI) and the action mechanism.Methods SD rats were divided into 3 groups.The model of unilateral renal IRI was established in SD rats,and Icariin (100 mg/kg) was orally administrated by gavage daily from 2 days before operation to 12 days after operation (icariin group).The vehicle of icariin was administrated to IRI model rats as control group,while in the sham-operation group the renal pedicel was only dissociated without treatment.Body weight and kidney function were monitored within 14 days after reperfusion.The kidney was harvested at 24 h after reperfusion,and then malonaldehyde (MDA) and activity of reactive oxygen species (ROS) scavenger enzymes were examined.Histopathological changes were observed at postoperative day (POD) 3 and 14.Results At POD 3,7,11 and 14,the creatinine clearance rate was significantly higher in icariin group than in control group (P＜0.01). Icariin group had significantly lower Paller scores which indicated tubules injury than in control group at day 3 after reperfusion (P＜0.01 ).In icariin group,MDA level was obviously decreased at 24 h after operation.Compared to control group,icariin group had statistically higher activity of glutathion reductase (GR),catalase and superoxide dismutase (SOD),as well as higher level of reduced glutathione (GSH) (P＜0.05).Conclusion Icariin has protective effects on renal IRI,and can promote recovery of kidney function. Icariin can reduce oxidative stress through increasing activity of ROS scavengers.