Studies on inhibitory effect of rapamycin on growth and metastasis of PC3 cells
10.3760/cma.j.issn.1008-1372.2012.06.012
- VernacularTitle:雷帕霉素抑制人前列腺癌细胞PC-3生长及转移的实验研究
- Author:
Shaofei LOU
;
Chuan ZHANG
;
Jingchao CAO
;
Guiying HOU
;
Ming YE
;
Yakun ZHAO
- Publication Type:Journal Article
- Keywords:
Sirolimus/pharmacology;
Prostatic neoplasms/metabolism/pathology;
Apoptosis;
Neoplasm metastasis
- From:
Journal of Chinese Physician
2012;14(6):759-762
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of rapamycin on the proliferation and cell cycle of prostate cancer cell lines PC3,and to investigate the mechanism that it inhibits the growth and metastasis of prostate cancer cells.MethodsPC3 cells were cultured in vitro,and treated with different concentrations (10,25ng/ml) of cycle raparnycine.MTT was used to measure the change of proliferation of PC3 cells.Flow cytometry was used to measure the changes of cell cycle and apoptosis of PC3 cells.Nude mice were used to detect the effect of rapamycin on metastasis.ResultsThe proliferation of PC3 cells was significantly inhibited by rapamycin,and a time- and concentration-dependent relationship was shown,the inhibited rate was(42.23 ± 0.78 ) ％ after 36 h in the group of 25 ng/ml ( P ＜ 0.05 ).Flow cytometry analysis showed rapamycin significantly inhibited the cell cycle,prompted the apoptosis,and increased the number of cells in G0/G1 phase at 36 h with a rate of cell staying at Go/G1 [ (92.17 ± 0.69 ) ％ ] ( P ＜ 0.05 ).The weight of tumors in nude mice in the control group was significantly greater than that in RPM group[ (3.41± 0.28)g vs ( 1.19 ± 0.23 )g] ( P＜ 0.05 ),and metastatic sites of the lung and liver in the control group were significantly mote than the RPM group [ 100％ (7/7) vs 14.29％ ( 1/7 ) ] ( P ＜ 0.05 ).Conclusions Rapamycin significantly inhibits the proliferation and metastasis of osteosarcoma.The rapamycin-based regimen is valuable for clinical application.
