Effect of sulforaphane on human small cell lung cancer NCI-H446 cells proliferation, invasion and the activity of MMP-9
10.3760/cma.j.issn.1008-1372.2012.05.012
- VernacularTitle:莱菔硫烷对人小细胞肺癌NCI-H446细胞增殖、侵袭以及MMP-9活性的影响
- Author:
Wang SHI
;
Chengping HU
;
Ke TIAN
;
Zhike XIAO
- Publication Type:Journal Article
- Keywords:
Isothiocyanates/pharmacology;
Small cell lung carcinoma/metabolism/pathology;
Cell line,tumor;
Cell proliferation;
Neoplasm invasiveness;
Matrix metalloproteinase 9/metabolism
- From:
Journal of Chinese Physician
2012;14(5):618-621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of sulforaphane (SFN) on proliferation and invasion of human small cell lung cancer NCI-H446 and the activity of matrix metalloproteinase (MMP) -9.Methods NCI-H446 cells were cultured with 0,25,50,100 μmol/L SFN for 24 ～ 72 h,then MTT assay was employed to detect cell proliferation.Chamber invasion assay was used to study the cell invasion,and gelatin zymography assay was implied in MMP-9 enzyme activity.Results After treatment of 25,50,100μmol/L SFN,the growth of NCI-H446 cells were inhibited.When cells were incubated with 25,50,100μmol/L of SFN for 72h,the inhibition ratio was ( 11.1 ± 2.26 ) ％,( 25.2 ± 3.24 ) ％ and ( 44.6 ±4.2) ％,respectively,the difference was statistically significant compared with the solvent control group ( t =10.685,8.417,5.264,P ＜0.05 ).Chamber invasion assay showed that NCI-H446 cell invasion could be reduced.25,50,100 μmol/L of SFN could decrease the trans-membrane cells to (48.6 ± 1.84)％,(35.4 ± 2.22) ％ and (27.8 ± 1.36) ％,and it was statistically significant compared with the solvent control group ( t =6.341,5.562,4.925,P ＜0.05 ),respectively.In addition,MMP-9 activity was significantly inhibited by SFN.25,50,100 μmol/L of SFN could decrease the gray value of MMP-9 to 764 ±18.4,685 ± 14.74 and 638 ± 21.54 ( control group 822 ± 12.53,t =4.971,7.582,11.235,respectively,P ＜0.05).Conclusions SFN can inhibit NCI-H446 cells growth,invasion and the activity ofMMP-9.
