Effects of grape seed proanthocyanidin extracts on advanced glycation end product receptor and connective tissue growth factor in the myocardium of diabetic rats
10.3760/cma.j.issn.0254-9026.2011.11.020
- VernacularTitle:葡萄籽原花青素对糖尿病大鼠心肌糖基化终末产物受体和结缔组织生长因子的影响
- Author:
Mei CHENG
;
Baoying LI
;
Qian WANG
;
Ling XU
;
Haiqing GAO
- Publication Type:Journal Article
- Keywords:
Vitis;
Proanthocyanidins;
Diabetes complications;
Glycosylation end products,advanced;
Fibroblast growth factor
- From:Chinese Journal of Geriatrics
2011;30(11):958-961
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of grape seed proanthocyanidin extracts (GSPE) on advanced glycation end product receptor (RAGE),NF-Κb and connective tissue growth factor (CTGF) in the myoeardium of diabetic rats.MethodsTotal 30 streptozotocin (STZ) induced diabetic rats were randomly divided into 2 groups:diabetic group (DM1,n-15) and GSPE (250 mg/ kg,i.g) treated diabetic group (DM2,n= 15).Another two control groups:normal rats(C1,n= 10)and normal rats treated with GSPE (250 mg/kg,i.g) (C2 group,n= 10) were also observed.After 24 weeks,blood was collected to measure fasting plasma glucose (FBG) and RAGE.The protein expression of NF-Κb was determined in myocardial tissue by immunohistochemical staining and Western blot.The protein expressions of RAGE and CTGF were measured by Western blot.Results The levels of FBG and RAGE were significantly higher in diabetic rats than in control rats (P＜0.05).After GSPE treatment,RAGE level significantly reduced (P＜0.05),but FBG had no change in diabetic rats.The protein expressions of RAGE,NF-Κb and CTGF in the myocardial tissue of diabetic rats had marked increase compared with control rats (P＜ 0.05),however,their levels significantly reduced after GSPE treatment (P＜0.05).ConclusionsGSPE may protect diabetic rats against cardiomyopathy,possibly by decreasing the protein expressions of RAGE,NF-Κb and CTGF.
