The neuroprotective effect of hyperbaric oxygenation on the mitochondria of dopaminergic neurons in a mouse model of Parkinson's disease
10.3760/cma.j.issn.0254-1424.2012.09.001
- VernacularTitle:高压氧对四氢吡啶诱导的帕金森病小鼠黑质多巴胺能神经元的线粒体保护作用研究
- Author:
Mingming MA
;
Xuejing WANG
;
Xuebing DING
;
Jiewen ZHANG
;
Wei LI
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygenation;
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine;
Caspase;
Tyrosine hydroxylase
- From:Chinese Journal of Physical Medicine and Rehabilitation
2012;34(9):641-645
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate any neuroprotective effect of hyperbaric oxygenation (HBO) on the mitochondria of dopaminergic neurons using a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson's disease.Methods Forty-eight male SPF C57BL/6 mice were randomly assigned to either a normal control group (treated with a 30 ml/kg intraperitoneal injection of physiological saline once a day),a model group ( treated with a 30 mg/kg intraperitoneal injection of MPTP once a day) or an HBO therapy group ( treated with a 30 mg/kg intraperitoneal injection of MPTP and HBO once a day).The expression of tyrosine hydroxylase (TH) protein,PINK1 protein and caspase-3 in brain tissue was measured using immunohistochemistry and Western blotting assays.Results Compared with the control group,the expression levels of TH protein and PINK1 protein were significantly lower in the neurons of the substantia nigra in the model mice.HBO therapy upregulated the expression of TH and PINK1 protein.Compared with the control group,the average level of caspase-3 protein in the neurons of the substantia nigra in the model mice was significantly higher.HBO therapy downregulates the expression of caspase-3 protein.Conclusions HBO can protect mitochondria and inhibit apoptosis of dopaminergic neurons in the substantia nigra of brains with (MPTP-induced) Parkinson's disease by upregulating the expression of PINK1 protein and TH protein,and downregulating the expression of caspase-3 protein.
