Influences of Maixiansan on insulin-like growth factor hinding protein7 and apoptosis in rats with ulcerative colitis-related colorectal cancer
10.3760/cma.j.issn.0254-9026.2012.04.018
- VernacularTitle:麦纤散对大鼠溃疡性结直肠癌胰岛素样生长因子结合蛋白7及细胞凋亡影响
- Author:
Haiping ZHONG
;
Suya LIU
;
Guofa LI
;
Huiqing LV
;
Hongbin ZHENG
- Publication Type:Journal Article
- Keywords:
Maixiansan;
Proctocolitis;
Colorectal neoplasms;
Apoptosis;
Insulin-like growth factor binding proteins
- From:
Chinese Journal of Geriatrics
2012;31(4):326-329
- CountryChina
- Language:Chinese
-
Abstract:
Objective To invcstigate the effects of Maixiansan on insulin-like growth factor binding protein 7 (IGFBP7) and apoptosis in rats with ulcerative colitis related colorectal cancer.Methods The rat model of ulcerative colitis-related coloreetal cancer was induced by dextran sulfate sodium (DSS) and azoxymethane(AOM). 40 male SD rats [weight (160 ± 10) g] were randomly divided into 4 groups: model, Maixiansan and Meisalazine treatment as well as normal group peritoneally irjected with saline.The expression of IGFBP7 and apoptosis in coloreetal tissue were detected by real-time PCR and TUNEL after 16 weeks. Results The numbers of colorectal cancer in model group( 1.2 ± 0.4 ),in Maixiansan group ( 0.70 ± 0.15 ),in Meisalazine group ( 0.60 ± 0.16 )were higher than in normal control (P ＜ 0.05), but no differences were found among model,Maixiansan and Meisalazine groups(P＞0.05).The apoptosis in colonic mucosa for Meixiansan(8.70±3.47) group and Mesalazine group were enhanced as compared with that in model group( 1.20 ±0.26 vs.0.38±0.11,P＜0.05).The mRNA expression of IGFBP7 in colon for Meixiansan group were higher than those in model group,Meisalazine group,and normal control(50.5 ± 14.0 vs.18.0 ±3.9 and 39.3±11.4,46.4±6.0,P＜0.05). Conclusions Maixiansan may resist the occurrence and development of ulcerative colitis-related colorectal cancer through upregulating IGFBP7 expression of colorectal tissue and promoting apoptosis of tumor cell.
