Expression and clinical significance of phosphorylation of mammalian target protein of rapamycin in human glioma
10.3760/cma.i.issn.1673-4904.2012.02.002
- VernacularTitle:磷酸化哺乳动物雷帕霉素靶蛋白在人神经胶质瘤中的表达及临床意义
- Author:
Gang CHENG
;
Junlong XU
;
Xueguang ZHANG
;
Xin LI
;
Yubo REN
;
Lianqun ZHANG
;
Cunyou ZHAO
;
Xueyuan LI
- Publication Type:Journal Article
- Keywords:
Glioma;
Proliferating cell nuclear antigen;
Apoptosis;
Mammalian target proteinof rapamycin;
Survivin
- From:
Chinese Journal of Postgraduates of Medicine
2012;35(2):3-7
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the association of phosphorylation of mammalian target protein of rapamycin (pmTOR) expression with glioma malignancy grades,and the correlation of pmTOR expression with Survivin and Ki-67,which represent tumor cell anti-apoptosis ability and reproductive activity.MethodsImmunohistochemistry EliVision method was employed to detect the expression of pmTOR,Survivin and Ki-67 in paraffin tissues from 87 patients with glioma (grade Ⅰ - Ⅱ 27 cases,grade Ⅲ24 cases and grade Ⅳ 36 cases).The association between positive expression rate,level of pmTOR and malignancy grades,and the correlation of its expression level with Survivin and Ki-67 were further evaluated.Results There was no significant difference in the positive expression rate of pmTOR among grade Ⅰ - Ⅱ(77.8％,21/27),grade Ⅲ(75.0％,18/24) and grade Ⅳ (72.2％,23/36) (P ＞ 0.05).However,the significant association between pmTOR expression level and malignancy grades was observed.The expression from 87 patients with glioma was significantly positively correlated with Survivin and Ki-67 expression level (r =0.858,P ＜ 0.01 ;r =0.708,P ＜ 0.01 ).ConclusionsThe expression level of pmTOR is associated with malignancy grades,tumor cell anti-apoptosis ability and reproductive activity.pmTOR may be served as a useful marker for predicting the biological behavior of glioma and a useful target for gene therapy.
