Analysis of chromosomal abnormality and Y chromosome microdeletions in Chinese infertile men
10.3760/cma.j.issn.1000-6702.2011.03.005
- VernacularTitle:中国男性不育患者染色体核型及Y染色体微缺失分析
- Author:
Qiyi HU
;
Qianjin FEI
;
Jian CAI
;
Zhiliang WENG
;
Lizhang LIN
;
Xuefeng HUANG
;
Chengdi LI
- Publication Type:Journal Article
- Keywords:
Infertility,male;
Chromosome disorders;
Y chromosome microdeletions
- From:
Chinese Journal of Urology
2011;32(3):160-163
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between chromosomal abnormality and Y chromosome microdeletions and male infertility. Methods Lymphocytes were cultured from peripheral blood of 1975 male infertility patients and stained with Giemsa. The chromosomes were analyzed under microscope. Y chromosome specific sequence tags (STS) were selected, then the Y chromosome microdeletions in AZF regions were screened by polymerase chain reaction (PCR) in azoospermia and oligozoospermia patients. Results There were 305 cases of detected chromosomal abnormalities (15.44%) in the 1975 cases. There were 101 cases (5.11 %) with autosome abnormalities which clinically manifested as oligozoospermia and teratospermia. There were 204 cases (10. 33%) of sexual chromosome abnormalities and the patients were mainly characterized with Klinefelter's syndrome. Y chromosome microdeletions were detected in 109 (14.97 %) of the 728 cases of azoospermia or oligozoospermia. The most common microdeletion of Y chromosome was AZFc (62.39%) and these patients were characterized with azoospermia and oligozoospermia. Five patients (4. 59%) who suffered Y chromosome microdeletion in AZFa region and AZFb region were characterized with azoospermia. Fifteen cases (13.76%) with microdeletion in AZFb region and AZFc region were mainly characterized with azoospermia. There were 6 cases (5. 50 % ) of microdeletion in AZFa, AZFb and AZFc regions,these patients were all characterized with azoospermia. Conclusions Both Chromosome abnormalities and Y chromosome microdeletions are important causes for male infertility.
