Effect of BDNF gene modified umbilical cord mesenchymal stem cells transplantation on neurological function improvement in rats subjected to brain trauma
10.3760/cma.j.issn.1674-6554.2011.02.007
- VernacularTitle:携带脑源性神经营养因子基因人脐带干细胞改善脑损伤大鼠神经功能的实验研究
- Author:
Yuan YUAN
;
Qiqin DAN
;
Jia LIU
- Publication Type:Journal Article
- Keywords:
Brain injury;
Umbilical cord mesenchymal stem cells( UCMSC);
Brain derived neurtrophic factor;
Neurological function improvement;
Rat
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(2):115-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of brain derived neurtrophic factor(BDNF) gene modified umbilical cord mesenchymal stem cells ( UCMSC ) transplantation on neurological functional improvement in rats after brain trauma.Methods Cerebral contusion model in motor-sensory cortex in rats was established by a weight hammer falling method.UCMSC were cultured and transferred with BDNF gene.After BDNF expression and activity were determined,the BDNF gene modified UCMSC were implanted into traumatic brain.The neurological function was evaluated for 2 weeks after brain injury.And the BDNF expression was determined by using immunohistochemistry.Results Severe neurological dysfunction was seen in animals that had been subjected to contusion brain injury( 10.50 ±0.53 ).A significant improvement on neurological function was found in the UCMSC transplantaion animal( 7.75 ± 0.71 ), compared with only brain injury group (P ＜ 0.01 ).Moreover, rats in BDNF gene modified UCMSC showed the most behavior improvement ( 5.50 ± 0.76 ) (P ＜ 0.01 ).Conclusion BDNF gene modified UCMSC transplantation can survive and migrate, and improve neurological function in brain traumatic rats.
