Genistein and daidzein promote cell proliferation and neurotrophin expression in hippocampal H19-7 cell line
10.3760/cma.j.issn.1674-6554.2011.05.007
- VernacularTitle:染料木素和大豆黄素对海马神经细胞H19-7增殖和神经营养因子表达的影响
- Author:
Meixia PAN
;
Victor YEUNG
;
Hongyu HAN
;
Qingshan GENG
;
Ruojun XU
- Publication Type:Journal Article
- Keywords:
Genistein;
Daidzein;
Hippocampus;
Brain-derived neurotrophic factor;
Proliferation
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(5):404-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of genistein and daidzein on the proliferation and survival of the hippocampal neural cells and underlying mechanism. Methods H19-7/IGF-IR neural cell line was cultured in phenol red free DMEM absented of serum for 72h. Genistein, daidzein or 17β-estradiol was added to the culture at various concentrations. Their proliferation and protective effects on the neuronal cells were determined by BrdU and MTT assay respectively. The effect of phytoestrogens on cell cycle regulation was determined using flow cytometry. The effects of the soy isoflavones on brain-derived neurotrophic factor (BDNF) expression were determined by ELISA and RT-PCR respectively. Results It was observed that, with 72h of treatment, 20nM and 200 nM 17B-estradiol significantly promoted the neuronal cell proliferation at 33% and 36% ;20nM and 200nM genistein significantly promoted the neuronal cell proliferation at 15% and 13% ; 200nM daidzein significantly promoted the neuronal cell proliferation at 11% compared to the control (P＜0.05). Genistein and daidzein induced an significantly increase in the S phase arrest at (17.64 ± 0.43) % and (19.48 ± 1.01) % compared to the control (P ＜ 0. 05). Moreover, genistein and daidzein significantly increased the expression of mature BDNF and BDNF mRNA level (P＜0.05). The effect of genistein and daidzein on hippocampal neuronal cell proliferation was blocked by K252a, selective inhibitor of tyrosine kinase receptors. Conclusion Genistein and daidzein improved hippocampal neuronal cell proliferation and viability in vitro. The effects might be mediated by increasing in BDNF expression.
