Effects of advanced glycation end-products on matrix metalloproteinase 2 expression in INS-1 cells

VernacularTitle:糖化终末产物对INS-1细胞基质金属蛋白酶2表达的影响
Author: Ning LIN ; Xiaoyu WAN ; Xueru CHEN ; Qing SU ; Yan DONG
Publication Type:Journal Article
Keywords: Glycation end-products,advanced; Oxidative stress; Matrix metalloproteinase 2
From: Chinese Journal of Endocrinology and Metabolism 2011;27(4):328-331
CountryChina
Language:Chinese
Abstract: Objective To explore the impact of advanced glycation end-products ( AGEs) on matrix metalloproteinase 2 ( MMP-2) expression in cultivated INS-1 cells. Method INS-1 cells were cultivated and MMP-2 expression was analyzed. Glycated serum was prepared for incubating with INS-1 cell. Reactive oxygen species (ROS) was detected by flow cytometry. The intracelluar MMP-2 expression was analyzed by RT-PCR, realtime PCR and Western blot. The MMP-2 cDNA was expressed in cultivated INS-1 cells. Result The level of ROS treated with AGEs was significantly higher than that in the control( P<0.05 ) , and the levels of MMP-2 and its protein expressions turned out as well( P<0. 05). Conclusion The results suggest that MMP-2 was expressed in INS-1 cells. Increased MMP-2 expression in ?cells may be induced by AGEs, suggesting that MMP-2 might play an important role in oxidative stress-mediated islet injury.