Sevoflurane Enhances γ-aminobutyric Acid Gated-chloride Current in Cultured Neuron from Rat Dorsal Root Ganglia
- VernacularTitle:七氟烷增强单个培养鼠背根神经节细胞γ-氨基丁酸调控的氯电流
- Author:
Ailin LUO
;
Sugiyama KAZUHIDE
- Publication Type:Journal Article
- Keywords:
sevoflurane;
γ-aminobutyric acid;
patch clamp technique;
chloride current
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2000;29(5):458-460
- CountryChina
- Language:Chinese
-
Abstract:
The whole-cell patch clamp technique and "Y-tube" method were applied to evaluate the effects of sevoflurane (from 0. 3×10-3to 3×10-3 mol/L) on chloride current induced by bath utilization of 3×10-6 mol/L γ-aminobutyric acid (GABA) in the single-cultured rat dorsal root ganglia neurons. Experimental data demonstrated that when peak amplitude of chloride current induced by 3 × 10-6 mol/L GABA was considered as 100 % in the presence of 0. 38×10-3, 0. 76×10-3, 2. 28×10-3, 3. 04×10 -3 mol/L sevoflurane peak amplitude of chloride current was rose to (149±25) %, (203±-27) %, (327±79) %, (331±109) %, (243±71) % correspondingly. This finding suggests that sevoflurane, at concentrations relevant clinical anesthesia, can enhance GABA-mediated chloride current in sensory neurons.
