Mouse atrial tumor myocytes AT-1 in cardiac electrophysiologic and pharmacologic studies
- VernacularTitle:小鼠心房肌瘤AT-1细胞在心脏电生理学和药理学研究中的应用
- Author:
Tao YANG
- Publication Type:Journal Article
- From:
Chinese Journal of Pharmacology and Toxicology
2001;15(2):105-110
- CountryChina
- Language:Chinese
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Abstract:
Atrial tumor myocytes (AT-1 cells) were derived from tumor-bearing mice in which SV40 T large antigen drives atrial natriuretic promoter. AT-1 cells possess many features typically seen in cardiac cells from other species, such as spontaneous beating, multiple ion channels and responses to drugs, etc. Major ion channels are present in AT-1 cells: sodium, calcium and potassium channels. Sodium channel in AT-1 cells is highly sensitive to blocker tetrodotoxin. L- and T-type calcium channels respond to classical blockers. An important outward potassium current in AT-1 cells is the rapidly-activating delayed rectifier (IKr). IKr Is the sole time-dependently repolarizing potassium current in AT-1 cells. AT-1 cells are a very useful tool for studies in cardiac electrophysiology and pharmacology. As yet, a great number of cardiac and non-cardiac drugs have been tested for their IKr blocking actions in AT-1 cells. The polymorphic ventricular arrhythmia (torsade de pointes) is a severe side effect of IKr blockers.
