Detection polymorphism of CYP1A1 gene by allele specific amplification-ELISA
- VernacularTitle:等位基因特异性扩增酶联免疫法检测CYP1A1基因多态性
- Author:
Xinwen ZHOU
;
Yun SHI
;
Yikai ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Clinical Laboratory Science
2001;19(3):133-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective For establishing a effective method of gene polymorphism. Methods Polymorphism of CYP1A1 were detected by allele specific amplification and allele specific amplification-ELISA in this study.Results The results of two testing methods were same except one sample.PCR products were labeled with Dig in ASA-ELISA,then the PCR products were detected by specific and sensitive ELISA.The assay detection was 100-fold more sensitive than gel detection of ASA PCR products.Conclusion It was simple and short time,but it was more expensive.
