Expression and Function Analysis of Cholinergic Components nAChRα7 in Preadipocytes
- VernacularTitle:非神经元型胆碱能系统在(前)脂肪细胞的表达及nAChRα7功能状态对前脂肪细胞Visfatin基因表达的影响
- Author:
Jing WU
;
Huiling LU
;
Xiufen HU
;
Xiaoyan LIANG
;
Hanhua LIN
;
Hongwei WANG
;
Yang YANG
;
Zhi XIA
- Publication Type:Journal Article
- Keywords:
non-neuronal cholinergic system;
cholinergic anti-inflammatory pathway;
visfatin;
inflammation;
obesity
- From:
Chinese Journal of Biochemistry and Molecular Biology
2008;24(9):803-809
- CountryChina
- Language:Chinese
-
Abstract:
The non-neuronal cholinergic system, widely exists in prokaryotic, eukarytic, and even plant cells, however, it has not been investigated in preadipocytes and adipocytes. To search for evidence its existence in preadipocytes and adipocytes, the nicotinic acetylcholine receptor (nAChR) α7 subunit, acetyicholinesterase (ACHE) and choline acetyltransferase (CHAT) in 3T3-L1 cells were examined using immunohistochemical staining and Western blotting. The choline-regulated visfatin expression in 3T3-L1 preadipocytes was also tested by reverse transcriptase-PCR. Incubation with methyilycaconitine (10-6 to 10-4mol/L) for 12, 24 and 36 hours dose-dependently increased visfatin expression from 1.3- to 1.55-folds (P <0.01) with maximal induction at 24 hours with 10-4mol/L methyllycaconitine. Nicotine treatments (10-6 to 10-4 mol/L) for 12, 24 and 36 hours decreased visfatin expression; choline chloride (10-4 mol/L))suppressed visfatin expression in 3T3-L1 preadipocytes at 36 hours by 1.64 to 2.03 fold (P < 0.05) which was more effective as compared with nicotine. It was concluded that α7 nAChR was expressed in 3T3-L1 preadipocytes and involved in visfatin expression.
