Involvement of Heme Oxygenase-1 in the Protective Actions of Curcumin against Ethanol-induced Oxidative Damage in Rat Primary Hepatocytes
10.3870/j.issn.1672-0741.2010.01.020
- VernacularTitle:HO-1在姜黄素拮抗乙醇所致肝细胞氧化损伤过程中的作用
- Author:
Yingying OUYANG
;
Ke LI
;
Shuang RONG
;
Ping YAO
;
Nianhong YANG
;
Xiufa SUN
;
Liegang LIU
- Publication Type:Journal Article
- Keywords:
curcumin;
heme oxygenase-1;
ethanol;
oxidative stress
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2010;39(1):82-86
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of heme oxygenase-1(HO-1)in the protective actions of curcumin against ethanol-induced oxidative damage.Methods Microsomal HO-1 enzyme activities were determined in rat primary hepatocytes pretreated by curcumin.AST,LDH release and hepatic oxidative/antioxidant status were measured with or without ZnPPⅨ/Hemin as a classic HO-1 inhibitor/inducer,respectively.Results Curcumin could induce the HO-1 expression and enzyme activity,which was correlated with antioxidant levels in hepatocytes.HO-1 induction reached a peak under administration of 15 μmol/L curcumin for 1 h.Conclusion HO-1 induction by curcumin is contributed to the heptoprotective effects against ethanol-induced oxidative damage.
