Ginsenoside-Rg1 protects human dermal fibroblasts against 8-methoxypsoralen plus ultraviolet A-induced photoaging through p53-dependent signaling pathways
- VernacularTitle:人参皂苷Rg1对细胞光老化模型中p53信号转导途径的影响
- Author:
Yingying GAO
;
Wenli LIU
;
Bingrong ZHOU
;
Wei LI
;
Dan LUO
- Publication Type:Journal Article
- Keywords:
ginsenoside-Rg1;
photoaging;
8-oxo-dG;
p53;
p21~(WAF-1);
p16~(INK-4a)
- From:
Chinese Pharmacological Bulletin
2010;26(3):383-387
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the roles of p53-dependent signaling pathways in the process of ginsenoside Rg1 protection against 8-methoxypsoralen(8-MOP) and subsequent ultraviolet A(UVA) irradiation induced photoaging model in human dermal fibroblasts(HDFs).Methods Photoaging model was established by 8-MOP/UVA in skin HDFs.Flow cytometry, enzyme cytochemistry, immunofluorescence and Western blot were employed.Results Pretreatment with ginsenoside Rg1 could significantly reduce the amount of UVA-generated 8-oxo-dG and relieve the photoaging representation.Compared with 8-MOP/UVA treatment group, pretreatment with Ginsenoside Rg1 could decrease the expression of SA β-galatosides(SA-β-Gal), and down-regulate the level of senescence associated proteins(p16 and p21).Conclusions Ginsenoside Rg1 has prominent dose-dependent antagonism on senescence of skin HDFs induced by 8-MOP/UVA, and its mechanism may be due to its antioxidation which reduces the production of photoproducts to protect telomere against abnormal shortening.
