Effects of cannabinoid on the expression of PKAC-β,c-fos and BDNF in cerebral cortex after ICH
- VernacularTitle:大麻素对脑出血大鼠皮质内PKAC-β、c-fos及BDNF表达量的影响
- Author:
Li ZHU
;
Zhi DONG
;
Guodong ZHANG
;
Dong LI
- Publication Type:Journal Article
- Keywords:
WIN 55,212-2;
BDNF;
PKAC-β;
c-fos;
ICH;
Ⅶ collagenase;
CREB
- From:
Chinese Pharmacological Bulletin
2010;26(4):535-538
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of cannabinoid (WIN55,212-2) on mRNA and protein expressions of PKAC-β,c-fos and BDNF in cerebral cortex after intracerebral hemorrhage(ICH)in rats.Methods ICH model of rats were made by Ⅶ Collagenase,which were injected by the intraperitoneal route,thirty minutes after the operations.The rats were killed for brain tissue as specimens with 24 hours.The localizations of PKAC-β,c-fos and BDNF were assessed by immunohistochemistry;The expressions of PKAC-β,c-fos and BDNF mRNA were detected by RT-PCR;The expressions of PKAC-β and BDNF protein were revealed by Western blot.Results WIN55,212-2 increased not only the levels of BDNF mRNA and protein,but also c-fos mRNA in ipsilateral cerebral cortex.However,it decreased the levels of PKAC-β mRNA and protein.PKAC-β,c-fos,and BDNF proteins were expressed on membrane of neurons,nucleus of neurons or the cytoplasm of glial cells respectively.Conclusion WIN55,212-2 induces the expression of BDNF in the cerebral cortex,which provides a theoretical basis for the treatment of cerebrovascular disease.
