The immunosuppressive effect of rapamycin on lymphocytic proliferation and DTH、GVHR
- VernacularTitle:国产雷帕霉素对小鼠淋巴细胞增殖和DTH、GVHR的免疫抑制作用
- Author:
Guowei XIA
;
Hanying JIANG
;
Suisheng XIA
- Publication Type:Journal Article
- Keywords:
Immunosuppressant;
Lymphocytic proliferation;
DTH;
GVHR
- From:Chinese Journal of Organ Transplantation
2000;21(6):350-352
- CountryChina
- Language:Chinese
-
Abstract:
Objective To manufacture rapamycin (RPM)-a new type immunosuppressant made in our country and investigate its immunosuppressive mechanism.Methods The effect of RPM on mouse splenocyte (Tc) proliferation induced by ConA,splenocyte (Bc) proliferation induced by LPS,DTH induced by DNCB and rat GVHR were studied.Results RPM obviously restrained the proliferation of Tc with IC50=1 nmol/L,significantly lower than IC50(10 nmol/L) of CsA (P＜0.05).RPM evidently repressed the proliferation of Bc with IC50=1 nmol/L significantly lower than IC50＞10 nmol/L of CsA(P＜0.05).RPM inhibited murine DTH to DNCB with ED50:1.8 mg/kg,significantly lower than ED50＞30mg/kg of CsA(P＜0.05).RPM controlled rat's GVHR with ED50=3 mg/kg,significantly lower than ED50≥30 mg/kg of CsA(P＜0.05).Conclusions RPM can inhibit lymphocytic proliferation and DTH,GVHR more markedly than CsA and they have combined efforts.
